The use of chemical analyses for water quality management has limitations in terms of efficiently managing
pollutants through various pollutants sources. For the purpose of identification, stable isotope ratio methods are
wildly used around world. The measurement technique of oxygen and nitrogen stable isotopes in water is employed
to establish the origin and pollution source in the water quality protection area in this study. The target
study areas, which are a water resource, is located in G area, Korea, and the analytical samples were collected
4 times per year at 5 sites. As a result, the assigned values of oxygen and nitrogen stable isotopes in water were
changed based on rainfall. The target area, water quality protection area, was estimated to be highly influenced
by soil from land, manure and sewage, and contaminated groundwater. According to the correlation between
oxygen and nitrogen stable isotope ratio, the study area is estimated to be influenced by pollution sources in
D site, where inflow was not large.