Molecular typing of somatic coliphages to determine their presence and survival in environmental waters |
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학술지명 International Water Association
저자 이희숙
발표일 2012-09-17
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Public health risks posed by enteric viruses in water have led to the search for reliable indicators of them in drinking and recreational waters. Culture and molecular methods are available to detect enteric viruses in water, but they are difficult, expensive and slow to yield results when attempting to detect all enteric viruses. Coliphages are viruses of E. coli which is a bacterial indicator of fecal contamination of water. Many waterborne pathogens are enteric viruses, so a bacterial indicator alone may be inadequate or unreliable as indicator for estimating enteric virus presence in contaminated water. Somatic coliphages have long been proposed as viral indicators in sewage and water because they are abundant and often outnumber male-specific coliphages and human enteric viruses (Kott, 1966, Dhillon et al., 1970; Dhillon and Dhillon, 1972; Moce-Llivina et al., 2005, Jofre, 2008). Somatic coliphages are heterogeneous, encompassing four families: Myoviridae, Siphoviridae, Podoviridae, Microviridae, and several genera, with differences in DNA content, size, structure, and life cycle, ranging from tailed, double-stranded DNA phages of the order Caudoviriales to the small, cubic Microviridae containing single-stranded DNA (The Bacteriophage, 2nd ed., Chapter 2, 2006). In order to identify a candidate somatic coliphage family for use as an indicator for the presence of human enteric viruses in sewage and water, we developed nucleic acid-based molecular methods to detec |