Presence, molecular characteristics and geosmin producing ability of Actinomycetes isolated from South Korean terrestrial and aquatic environments |
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학술지명 Water Science and Technology
저자 이경희,김용연,최재원,최일환,김민정,오성애,이규철,김윤석,박종근,정점규
발표일 2011-07-06
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The unpleasant odor of drinking water is one of the major problems in many water utilities in the world. Actinomycetes have long been associated with odorous compounds. Considering the paucity of research on Actinomycetes producing odorous compounds in South Korea, presence of Actinomycetes, their molecular characteristics and ability to produce odorous compounds were investigated in this study. Findings confirmed the presence of Actinomycetes in surface soil, sediment, and water samples from four sites: two artificial lakes (PD and CP), and two streams (GA, YP). Surface soil and sediment from CP area had the greatest concentration of Actinomycetes (8.2×107 and 6.8×106 colony forming units (CFU)/gram, dry weight, respectively). When water samples are considered, samples from GA had the highest concentration (1.9×102 CFU/mL). 16S rRNA sequencing and molecular phylogenetic analysis showed that Streptomyces was the dominant genus (64.1%). In addition, the isolated Actinomycetes synthesized 5.4 ng/L geosmin as demonstrated by TDU-GC/MS analysis. |