논문실적
| 배양조류 및 댐저수지 조체 중 신경독소 Saxitoxin, Neosaxitoxin의 분석 |
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학술지명 한국환경분석학회
저자 김학철,이상태,정유진,최일환
발표일 2008-06-16
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In this study we set up the analytical methods for the determination of saxitoxin and neosaxitoxin using HPLC/FLD system. This analytical methods were applied to algae culture and algae extracts. For the HPLC/FLD analysis of saxitoxin and neosaxitoxin samples were separated on the column and then derivatizied by post column reactor for fluorescen detection. For post column reaction of saxitoxin we feed two kinds of reaction solution; Oxidizing Reagent of which composition was periodic acid(7mM) in 50mM potassium phosphate buffer, pH 9 and acidifying reagent of which Composition was 0.5M acetic acid. The LOD values for saxitoxin and neosaxitoxin in HPLC/FLD method were 35 ㎍/L, 27 ㎍/L respectively. We analyzed saxitoxin and neosaxitoxin in algae culture media and extracts of lypopyllized algal cell cultured and that of Deachung reservior. |
